Your toll on the Florida Turnpike would depend upon exactly where you get on in Orlando and where you get off in the Miami area, but a straight-through drive on the Turnpike between those two cities would cost you around $18 in cash. I-95 is free, but there is a $5 toll for the Beeline Expressway between Orlando and I-95. Since it is free, I-95 carries more traffic.
Going directly from Orlando to Miami via the Turnpike could be done in about three and a half hours (plus any stops for fuel/food/breaks) while heading to the coast and using I-95 would add between 20-45 minutes to that. Any time spent on FL-A1A should be counted as 'extra' in addition to the times already stated as this road will be very slow going. It is the local beach road everywhere with lots of tourist traffic, stop lights, cross traffic, and very low speed limits.
AZBuck